Germline Transcriptomics in Zebrafish
2014 – Present | Uppsala University, Uppsala, Sweden
My current post-doctoral research work falls within the framework of sexual evolution and the consequences of sex. One of the critical aspects is the role of germline selection in animals. The project involves identification of developmental stages during spermatogenesis in male zebrafish followed by analysis of their transcriptomes. We are interested in the genomic and as well as non-genomic effects of selection on the haploid germ cells. How do germline transcriptomes change during sperm development? What role does coding and non-coding RNAs play during the various stages? What sort of RNAs are packaged into a mature sperm and what is their role in fertilisation/post-fertilisation? What is the relationship between the transcriptome and the methylation pattern in the sperm genome? These are some of the research questions at hand. Learn more about our work and other ongoing projects at the Immler Lab (Evolutionary Biology Centre) in Uppsala.
Honey Bee Research
2009 – 2013 | Aarhus University, Slagelse, Denmark
I started working on European honey bees (Apis mellifera L.) as part of my PhD work. This included viral diseases, diagnosis and quantification. I have also been involved in colony monitoring and inspection as part of a EU project. Later, I worked on the population structure and spatial distribution of honey bee subspecies in Europe.
Characterisation of Chitinase genes from bacterium Pantoea dispersa
2008 – 2009 | University of Hertfordshire, Hatfield, UK
Chitin is a biopolymer found in many organisms such as arthropods and insects as well as in plants and fungi. Chitinases are enzymes that break down chitin. Chitinases are produced by a wide range of organisms, and their composition and functional properties may differ. In nature, chitin is part of the exoskeleton in insects, the cell wall of fungi or even a defensive mechanism in plants. Chitin and chitinases have extensive use in agriculture and industry including medical applications, food processing, fertilizer and nanomaterial research. Therefore, it is important to study the activity and stability of these enzymes using molecular approaches. A novel of class of chitinase genes were discovered in bacterium Pantoea dispersa. This project involved bacterial culture followed by identification, amplification, sequencing and sequence comparison of the chitinase genes in this bacteria.
Lux-integrated bio-luminescent Pseudomonas aeruginosa as a biosensor
2007 – 2008 | Master’s Project | University of Hertfordshire, Hatfield, UK
Microbes such as bacteria can be used as biosensors for a range of agricultural, environmental and industrial applications from monitoring toxicity in water bodies, detection of pathogens and contamination in industrial effluents. One particular application is the testing of pharmaceutical products for microbial activity. The traditional approach using viable count technique is time consuming, expensive and laborious. An alternative approach to bacterial quantification is proposed using measurement of light intensity in the samples using bioluminescent bacteria. This project involved the transformation of bacterium Pseudomonas aeruginosa with a pBR322 plasmid containing the bioluminescent lux gene construct.